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ABSTRACT
In-Vitro Comparison of Cytotoxic Effects of Two Zinc Oxide Eugenol Containing Root Canal Sealers on Human Periodontal Ligament Fibroblasts
Mrunal Vijay Deshmukh
ABSTRACT
Objective: To evaluate and compare the cytotoxicity of two zinc oxide eugenol (ZOE) based root canal sealers—Endoflux (Ammdent, India) and Endoseal (Prevest DenPro, India)—on cultured human periodontal ligament fibroblasts (hPDLFs). Materials and Methods: Primary human periodontal fibroblasts were cultured and exposed to freshly mixed and set specimens of the two ZOE sealers. Cell viability was assessed at 24 h, 48 h, and 72 h using the MTT assay. Data were statistically analyzed using one-way ANOVA followed by Tukey’s post-hoc test (p < 0.05). Results: Both ZOE sealers demonstrated time-dependent reduction in cytotoxicity. Endoseal exhibited significantly higher cell viability at all time intervals compared to Endoflux (p < 0.05). Cytotoxicity decreased after setting in both groups. Conclusion: Endoseal (Prevest DenPro) demonstrated comparatively lower cytotoxic effects on human periodontal fibroblasts than Endoflux (Ammdent), suggesting a more favorable biocompatibility profile for clinical use.
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