Journal of Applied Dental
and Medical Sciences
Journal of Applied Dental and Medical Sciences is A Quarterly Published


Genotoxicity evaluation of Sixth, Seventh and Eighth generation dentin bonding agents by single cell gel electrophoresis technique in human lymphocytes

Krishnaprasad shetty, Satish S.V. , Krishnarao kilaru , Angel Bhagya, Basavana Gouda, Sneha latha patil, Alexander M Luke


The main aim of introducing dentin bonding agents is to enhance the bonding of composite resins to dentin as well as minimizes the microleakage at tooth restoration interface. Dentin bonding agents have different chemical compositions, mechanism of action, and clinical application procedures. Therefore different biological effect on the pulpal tissues, could be ranged from severe to none it again depends on several other factors. The use of dental adhesives in routine dental practice has raised questions about their biologic safety. Since, several brands and generations of bonding agents are now available based on clinical use and implication, but their biocompatibility could be a relevant aspect of the clinical success of those materials. Objectives: To evaluate the genotoxicity of three different dentin bonding agents Clearfil SE Bond, Gluma self-etch, Futura Bond by comet assay.Methods: Genotoxicity evaluation of different dentin bonding agents was carried out in vitro in human lymphocytes at different elution concentrations using the comet assay. Statistical comparison of the results was carried out by ANOVA. Results: One of the tested dental adhesives i.e, Clearfil Se bond revealed a statistically significant increase in the tail length, tail intensity or tail moment in treated lymphocytes, independent of the dose related. A slight increase in the tail length and intensity of DNA molecules was observed in Gluma and Futura bond of the elution period at the lowest dilution. Conclusion: under the conditions used in this study, all adhesives had acceptable biocompatibility in terms of genotoxicity.

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