Nikhil Mahanubhav, Tarun Ahuja, Zinnie Nanda, Kranthikumar Reddy, Juili Gawande, Prasad Rane

ABSTRACT

Background: EDTA is used as agent of choice in preparation of canals, not many studies have been performed previously to find a substitute for EDTA. The aim of the study is to examine the chelating effects of EDTA and two additional agents’ viz. Citric acid and Deferoxamine. Objective: To compare the effect of three chelating agents on root canals of extracted sound human teeth. Method: 30 extracted non- carious single rooted teeth will be included in the study. Rotary instrumentation was performed with rotary files in a crown down fashion to a standardized master apical file #30.Teeth will be irrigated with 5 ml diiodized water and divided into 3 groups, 10 specimens in each. Each group will be treated separately with 1ml of chelating agent as follows. Group 1 – treated with 1 ml of 17 % EDTA for 5 min. Group 2 – treated with 1 ml of 10 %citric acid for 5min. Group 3 – treated with 1 ml of 10 % deferoxamine for 5 min. The teeth will be sectioned longitudinally. The two halves will be separated by a chisel to avoid touching the pulp chamber. The teeth will be mounted and put in a vacuum chamber for 2 weeks to dehydrate the sections .The middle third of root canals will be scanned at x 1000 using a JEOL,JSM-840 A under SEM. Result: All the values will be recorded and subjected to statistical analysis.